Figure S2.

Agreement of rupture force distributions across biological replicates. (A) Superplot showing the rupture forces measured using beads coated with Ndc80 complex alone (in the absence of free Dam1 complex), colored separately for the first biological replicate (aquamarine), the second biological replicate (orange), and the third biological replicate (only for wild type; blue). Data points above the horizontal dashed line were right-censored, when a bead reached the maximum trap force before rupturing. Larger colored symbols represent medians and 95% CIs for each biological replicate. Black symbols represent medians and 95% CIs for all the data combined and are identical to the medians shown in Fig. 1 C. (B) Superplot showing rupture force measured using beads coated with Ndc80 complex in the presence of 30 nM free Dam complex in solution. Aquamarine data were collected using the first biological replicate of both the Ndc80 and Dam1 complexes. Orange data were collected using the first biological replicate of the Ndc80 complex and the second replicate of the Dam1 complex. Blue data were collected using the second biological replicate of Ndc80 complex and the first biological replicate of Dam1 complex. Tan data were collected using the second biological replicate of both Ndc80 and Dam1 complexes. Data points above the horizontal dashed line were right-censored, when a bead reached the maximum trap force before rupturing. Larger colored symbols represent medians and 95% CIs for each biological replicate. Black symbols represent medians and 95% CIs for all the data combined and are identical to the medians shown in Fig. 1 C.