Figure 10.

ERF.F12 repression of fruit ripening requires HDA1 activity. A, Global H3K9Ac and H3K27Ac acetylation levels in WT, HDA1-OE, and HDA1-RNAi fruits at 41 DPA. Band intensities were normalized relative to total histone H3 loading controls. B, ChIP analysis of H3K9Ac and H3K27Ac levels at the ACS2, ACS4, PG2a, and PL promoters in WT, ERF.F12-OE-A, and ERF.F12-RNAi-B fruits at 41, 43, and 47 DPA. Data are shown as means ± sd with six biological replicates. C, TSA treatment inhibits promotion of fruit ripening in ERF.F12-RNAi lines. Fruits were infiltrated with 10-μM TSA at 36 DPA. The photographs were taken 7 days after infiltration. D, Relative transcript levels of ACS2, ACS4, PG2a, and PL 5 days after TSA treatment. Each value represents the mean of six biological replicates. Asterisks indicate statistical significance by Student’s t test, P < 0.05.