Fig. 2.

Nearest-neighbor assisted unsupervised interrogation of the current cohort cases. (a) T-distributed stochastic neighbor embedding (t-SNE) analysis of in-house 1258 cases together projected on Capper et al. 2801 samples (reference set). Reference tumors are shown in gray dots, the 1258 consultative cases are colored by final integrated diagnosis of tumor entities and shaped by their classifier scores: high-confidence scores >0.84 (square), suggestive scores between 0.3 and 0.84 (triangle), and not contributary scores <0.3 (cross). (b–d) Case study of low classification score cases Q727 (b) and P644 (c) as well as tumor of a class that is absent from the classifier: spinal ependymoma with MYCN amplification (SP-EPN-MYCN) (d).