Fig. 4.

Tumor purity is significantly associated with classifier classification confidence score. (a) Comparison of the classifier classification score with the DNA input amount used for methylation profiling. (b) Comparison of IDH1/2 and TERT mutation allele/variant frequency-based tumor purity with 3 methylation-based tumor purity estimation methods from RF_purity in IDH-mutated gliomas and TERT-mutated glioblastomas. (c) Correlation of the tumor purity and the classifier classification score in pre-classifier GBMs. Tumor purity is estimated using RF_purity-ABSOLUTE method. (d) Tumor purity in different classification score groups across different tumor entities. (e) Tumor purity and classifier score groups of the recently discovered spinal ependymoma with MYCN amplification subtype (SP-EPN-MYCN), which is unrepresented in the classifier, compared with other ependymoma subtypes represented in the classifier.