Fig. (4).

Human olfactory ensheathing cell (hOEC)-EVs protect neural progenitor cells (NPCs) from apoptosis. (A) Appearance of NPCs with different treatments. t-BHP caused a large amount of cell death. Scale bars: 200 µm. (B) The cell number was counted to evaluate cell damage after t-BHP treatment. (C) TUNEL assays were performed to count apoptotic cells after treatment. Quantification of TUNEL-positive cells was calculated as a percentage. (D) Lactate dehydrogenase (LDH) assay indicating cytotoxicity after t-BHP treatment and the alleviating effect of hOEC-EVs. (E) Representative western blot images of apoptotic protein expression. (F) Densitometric bar graph quantified from the three western blot replicates. *p<0.05, **p<0.01, ***p<0.001 (A higher resolution / colour version of this figure is available in the electronic copy of the article).