Fig. 5.

TAMs in human GBMs segregate based on compartment of origin. (A) UMAP plot of 10× Genomics scRNA-seq and snRNA-seq of TAMs from human GBM datasets clustered using Seuratv3. (B) Heatmap of marker genes for BAMs, monocyte-like cells, microglia, and proliferating TAMs from human GBMs. (C) UMAP plots of TAM clusters and percentages of BAMs, monocyte-like cells, microglia, and proliferating TAMs in different human GBM subtypes. (D) Rich plot of Hallmark gene sets enrichment in human BAMs vs microglia. (E) Correlation of mouse RAS-BAM and PDGFB-microglia signatures with TAMs in human GBMs. Abbreviations: BAMs, border-associated macrophages; GBM, glioblastoma; PDGFB, platelet-derived growth factor subunit B; TAMs, tumor-associated macrophages/microglia; UMAP, Universal Manifold Approximation and Projection.