Table 1.
Organoid and Parental Tumor Characteristics and Demographics
| Sample ID | Age | Sex | Brain Region | WHO Grade | Diagnosis | Primary/Recurrent | IDH Mutation | Viable | Tumor DNA | Organoid DNA | Sequenced (weeks) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| UTSW2591 | 55 | F | RF | 4 | GBM, IDH-wildtype | Primary | N/A | Y | Y | Y | 4 |
| UTSW1167 | 38 | F | LF | 2 | Astrocytoma, IDH-mutant | Primary | IDH1 R132H | Y | Y | Y | 4 |
| UTSW9534 | 55 | M | RF | 3 | Astrocytoma, IDH-mutant | Recurrent | IDH1 R132H | Y | N | N | N/A |
| UTSW8546 | 42 | F | LP | 3 | Astrocytoma, IDH-mutant | Primary | IDH1 R132S | Y | N | N | N/A |
| UTSW1164 | 58 | M | LT | 4 | GBM, IDH-wildtype | Primary | N/A | Y | Y | Y | 4 |
| UTSW3968 | 69 | M | LT | 3 | Astrocytoma, IDH-mutant | Primary | IDH1 R132H | Y | Y | Y | 4 |
| UTSW3762 | 58 | M | LP | 3 | Oligodendroglioma, IDH-mutant, 1p/19q co-deleted | Recurrent | IDH1 R132H | Y | Y | Y | 4 |
| UTSW6577 | 32 | M | RP | 4 | Astrocytoma, IDH-mutant | Recurrent | IDH1 R132H | Y | Y | Y | 4 |
| UTSW1382 | 27 | M | LF | 3 | Oligodendroglioma, IDH-mutant, 1p/19q co-deleted | Recurrent | IDH2 R172K | Y | Y | Y | 4 |
| UTSW2243 | 53 | F | LT | 4 | GBM, IDH-wildtype | Primary | N/A | Y | Y | Y | 4 |
| UTSW9647 | 33 | M | RF | 3 | Astrocytoma, IDH-mutant | Primary | IDH1 R132H | Y | N | N | N/A |
| UTSW1336 | 63 | F | RF | 2 | Oligodendroglioma, IDH-mutant, 1p/19q co-deleted | Recurrent | IDH1 R132H | Y | N | N | N/A |
| UTSW6992 | 51 | M | RF | 2 | Astrocytoma, IDH-mutant | Primary | IDH1 R132H | Y | N | N | N/A |
| UTSW3141 | 44 | F | LT | 2 | Oligodendroglioma, IDH-mutant, 1p/19q co-deleted | Primary | IDH1 R132H | Y | N | N | N/A |
| UTSW6511 | 28 | F | LT | 1 | PA | Primary | N/A | Y | N | N | N/A |
| UTSW2564 | 73 | F | RF | 4 | GBM, IDH-wildtype | Primary | N/A | Y | Y | N | N/A |
| UTSW8663 | 61 | F | LF | 4 | GBM, IDH-wildtype | Recurrent | N/A | Y | Y | N | N/A |
| UTSW7476 | 77 | F | LT | 4 | GBM, IDH-wildtype | Primary | N/A | Y | N | N | N/A |
| UTSW4699 | 57 | M | RF | 3 | Oligodendroglioma, IDH-mutant, 1p/19q co-deleted | Recurrent | IDH1 R132H | Y | N | N | N/A |
| UTSW4588 | 27 | M | LP | 3 | Oligodendroglioma, IDH-mutant, 1p/19q co-deleted | Recurrent | IDH2 R172K | Y | N | N | N/A |
| UTSW4432 | 40 | M | RF | 3 | Astrocytoma, IDH-mutant | Primary | IDH1 R132H | N | N | N | N/A |
| UTSW2933 | 32 | M | RF | 3 | Astrocytoma, IDH-mutant | Primary | IDH1 R132H | N | N | N | N/A |
List of primary tumor specimens and organoid models in the study. “Brain Region” column specifies location of the tumor at resection, abbreviations: RF, right frontal; LF, left frontal; LP, left parietal; RP, right parietal. “Diagnosis” column abbreviations: GBM, glioblastoma; PA, pilocytic astrocytoma. IDH status of each primary tumor sample was assessed via IHC, genomic DNA sequencing, or both. “Tumor DNA” and “Organoid DNA” columns indicate whether sequencing was performed. “Sequenced” column indicates the number of weeks following tumor explantation at which organoids were fixed for sequencing analysis. “Viable” column denotes organoid structural integrity at 4 weeks. UTSW2243 was sequenced but failed to meet quality assurance standards and sequencing results were omitted from further analysis. For all columns, Y = Yes, N = No, N/A = not applicable.