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Effect of PIC on COX-2 expression levels. (A) Protein expression levels of COX-2 were analyzed via western blotting. (B) Semi-quantification of COX-2 protein expression levels. (C) COX-2 mRNA expression levels were analyzed using reverse transcription-quantitative PCR. (D) IL-6, IL-1β and PGE2 levels were quantified via ELISA. n=3. ##P<0.01 vs. control; and **P<0.01 vs. TNF-α. PIC, piceatannol; COX-2, cyclooxygenase-2; PGE2, prostaglandin E2.
