Figure 1.

NPY1R receptor antagonist (BIBP-3222) is protective in DSS colitis. A, Mice were administered dextran sodium sulfate (3%) in normal drinking water for 1 week. On days 2, 4, and 6, mice also received intracolonic injections of NPY1R antagonist (BIBP-3222) or NPY2R (BIIE-0246) at a dosage of 10 mg/kg body weight. Mice were monitored daily for body weight, occult blood, and diarrhea. Mice were killed humanely on day 8, and colon length was assessed. Distal colon was paraffin embedded and sections were stained with hematoxylin eosin (H&E) to assess histological damage. B, Change in body weight. C, Clinical score. D, Representative histological H&E sections of colon showing crypt damage, ulcers and neutrophil infiltration. E, Graphical representation of the intensity of histological damage by histological score. F, Endoscopy images on day 8. G, Endoscopic colitis score. Magnification 20X, Scale bar 50 µm. Values are mean ± SEM from 2 independent experiments, n = 3 per group per experiment, n = 3-4 for endoscopy assessment, *One-way ANOVA followed by Dunnett multiple comparison test, P < .05, **P < .01, ***P < .001.