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. 2021 Oct 6;28(4):502–513. doi: 10.1093/ibd/izab243

Figure 2.

Figure 2.

NPY1R receptor antagonist (BIBP-3222) downregulates colonic TNF mRNA in DSS colitis. Mice were administered dextran sodium sulfate (3%) in normal drinking water for 1 week. On days 2, 4, and 6, mice also received intracolonic injections of NPY1R antagonist BIBP-3222 or BIIE-0246 (10 mg/kg body weight). Mice were killed humanely on day 8, and colons were flash frozen for RNA extraction. The colonic transcripts were assessed for changes in the expression of NPY, TNF, NPY1R, NPY2R by real time PCR (using primers given in Supplemental Table 1). Changes in (A) NPY, mRNA (B), TNF mRNA (C), NPY1R mRNA (D), NPY2R mRNA, and (E) NPY2R:NPY1R ratio. Values are mean ± SEM from 2 independent experiments, n = 6 (n = 3 per group per experiment). One-way ANOVA followed by Dunnett multiple comparison test, *P < .05, ***P < .001.