KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-GPR17 (1:200) | Novus | Cat#NLS4229; RRID: AB_2232678 |
| Mouse monoclonal anti-Chromogranin A (1:200) | Santa Cruz Biotechnology | SC-393941; RRID: AB_2801371 |
| Mouse monoclonal anti-GIP (1:100) | Abcam | Cat# ab30679, RRID: AB_2109682 |
| Mouse monoclonal anti-GLP1 (1:50) | Novus | Cat# NBP1–50697, RRID: AB_11016296 |
| Mouse monoclonal anti-HA (1:1000) | BioLegend | Cat# 901513, RRID: AB_2565335 |
| Goat anti-Rabbit IgG (H+L) (1:500) | Thermo Fisher Scientific | Cat# A-21428, RRID: AB_2535849 |
| Goat anti-Mouse IgG (H+L) (1:500) | Thermo Fisher Scientific | Cat# 35502, RRID: AB_844397 |
|
Biological samples | ||
| Human gut tissue | IU Health Methodist Hospital | NA |
|
Chemicals, peptides, and recombinant proteins | ||
| Aprotinin | Sigma-Aldrich | A6279; CAS: 9087–70-1 |
| DPP-IV inhibitor | Millipore | Cat#DPP4–010 |
| Exendin-3 (9–39) amide (Ex9) | Tocris | 2081; CAS: 133514–43-9 |
| Forskolin | Tocris | 1099; CAS: 66575–29-9 |
| IBMX | Sigma-Aldrich | I7018; CAS: 28822–58-4 |
| MDL29,951 | Laboratory of Donald Landry and Andrew Wasmuth | NA |
| Pertussis toxin (PTX) | Sigma-Aldrich | P2980; CAS: 70323–44-3 |
| Tamoxifen | Sigma-Aldrich | T5648; CAS: 10540–29-1 |
| Tetrodotoxin (TTX) | Tocris | 1069; CAS: 18660–81-6 |
| Y27632 | STEMCELL Technologies | Cat#72302 |
| NT(8–13) | Sigma-Aldrich | N5266; CAS: 60482–95-3 |
|
Critical commercial assays | ||
| GLP-1 ELISA kit | Meso Scale Discovery | Cat#K1503PD-1 |
| GIP ELISA kit | Millipore | Cat#EZRMGIP-55K |
| Insulin ELISA kit | Millipore | Cat#EZRMI-13K |
| TRUPATH kit | Gift from Bryan Roth | Addgene#1000000163 |
|
Deposited data | ||
| Mouse Fecal Microbiome Data | This paper | SRA: PRJNA756295 |
| IHC raw images and quantified data | This paper | https://doi.org/10.7912/D2/22 |
|
Experimental models: Cell lines | ||
| HEK293 | ATCC | CRL-1573 |
| GLUTag | Gift from Sonia M. Najjar | NA |
| HELA | ATCC | CCL-2 |
|
Experimental models: Organisms/strains | ||
| Mouse: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | The Jackson Laboratory | CAT#007909 |
| Mouse: B6.Cg-Tg(Vil1-cre)997Gum/J | The Jackson Laboratory | CAT#004586 |
| Mouse: B6;129S-Gcgtm1.1(cre/ERT2)Gkg/J | The Jackson Laboratory | CAT#030681 |
| Mouse: B6.Cg-Tg(Vil1-cre/ERT2)23Syr/J | The Jackson Laboratory | CAT#020282 |
| Mouse: Gpr17fl/fl | Columbia University Transgenic Mouse Core Facility | NA |
|
Oligonucleotides | ||
| See Table S1 for a list of all primers and oligonucleotides | This paper | NA |
|
Recombinant DNA | ||
| pcDNA3.1 (2021(−) | (Conley et al., 2021) | NA |
| pcDNA3.1-mGpr17-GFP | (Conley et al., 2021) | NA |
| pcDNA3.1-HA-hGPR17 | (Conley et al., 2021) | NA |
| pcDNA3.1(+)-hNT1R | CDNA | NTSR100000 |
| pcDNA3-HA-mGPR17 | This paper | NA |
| pGloSensor™-22F | Promega | E2301 |
|
Software and algorithms | ||
| pClamp 10.7 | Molecular Devices | NA |
| Prism 9 | GraphPad | NA |