TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| E. cloacae | ||
| GN7471 | Clinical isolate from Japan, resistance to cephaloridine and cefotiam | 30 |
| KU3261 | Clinical isolate from Japan | This study |
| KU3262 | Clinical isolate from Japan | This study |
| KU3263 | Clinical isolate from Japan | This study |
| ATCC 13047 | Purchased from ATCC; type strain, RF4738 | |
| E. coli | ||
| ML4947 | F−galK2 galT22 hsdR hsdM lacY1 metB1 relA supE44 RIFr | 13 |
| ML4953 | F−ampD9 galK2 galT22 hsdR hsdM lacY1 metB1 relA supE44 RIFr | ampD9 is deb9 (27) |
| Plasmids | ||
| pMS161 | 8-kb EcoRI fragment containing ampC and ampR from GN7471 cloned into pACYC184 | This study |
| pKU403 | 6-kb SalI fragment containing ampC and ampR from pMS161 cloned into pMW218 | This study |
| pKU404 | Mutant from pKU403 selected with aztreonam | This study |
| pKU405 | Mutant from pKU403 selected with ceftazidime | This study |
| pKU406 | Mutant from pKU403 selected with aztreonam | This study |
| pKU407 | Mutant from pKU403 selected with ceftazidime | This study |
| pKU408 | Prepared by deleting 1.7-kb SphI fragment containing ampR from pKU403 | This study |
| pKU409 | Prepared by deleting 1.7-kb SphI fragment containing ampR from pKU404 | This study |
| pKU410 | Prepared by deleting 1.7-kb SphI fragment containing ampR from pKU405 | This study |
| pKU414 | Prepared by deleting 1.7-kb SphI fragment containing ampR from pKU406 | This study |
| pKU411 | Prepared by deleting 4-kb BamHI-ScaI fragment containing ampC from pKU403 | This study |
| pKU412 | Prepared by deleting 4-kb BamHI-ScaI fragment containing ampC from pKU404 | This study |
| pKU413 | Prepared by deleting 4-kb BamHI-ScaI fragment containing ampC from pKU405 | This study |
| pKU415 | Prepared by deleting 4-kb BamHI-ScaI fragment containing ampC from pKU406 | This study |
| pACYC184 | Cloning vector, purchased from Nippon Gene (Tokyo, Japan); CPr TCr | 5 |
| pMW218 | Cloning vector, purchased from Nippon Gene (Tokyo, Japan); KMr | 3 |
a
RIF, rifampin; CP, chloramphenicol; TC, tetracyclin; KM, kanamycin; r, resistance; ATCC, American Type Culture Collection.