TABLE 2.
MICs for E. coli mutants
| Host | Plasmid | Statusa | MIC
(μg/ml)b
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ABPC | CER | CTM | LMOX | CTX | CAZ | CPDX | CFPM | AZT | IPM | |||
| ML4947 (AmpD wild type) | pKU403 | Wild type | 16 | 128 | 2 | 0.125 | 0.125 | 0.25 | 1 | 0.03 | 0.06 | 0.5 |
| pKU404 | H-mut | >128 | >128 | >128 | 8 | 32 | 64 | >128 | 0.25 | 32 | 1 | |
| pKU405 | L-mut | >128 | >128 | >128 | 4 | 16 | 32 | >128 | 0.125 | 16 | 2 | |
| pKU406 | I-mut | >128 | >128 | >128 | 2 | 8 | 8 | 128 | 0.06 | 4 | 2 | |
| pKU407 | I-mut | >128 | >128 | >128 | 2 | 8 | 8 | 128 | 0.06 | 4 | 2 | |
| pKU408 | pKU403 ΔampR | 8 | 16 | 2 | 0.125 | 0.125 | 0.25 | 2 | 0.03 | 0.125 | 0.5 | |
| pKU409 | pKU404 ΔampR | 16 | 16 | 4 | 0.125 | 0.125 | 0.25 | 2 | 0.03 | 0.125 | 0.5 | |
| pKU410 | pKU405 ΔampR | 8 | 8 | 2 | 0.125 | 0.125 | 0.25 | 2 | 0.03 | 0.06 | 0.25 | |
| pKU414 | pKU406 ΔampR | 8 | 16 | 4 | 0.125 | 0.125 | 0.25 | 2 | 0.03 | 0.125 | 0.25 | |
| pKU411 | pKU403 ΔampR | 8 | 2 | 0.125 | 0.125 | 0.06 | 0.125 | 0.5 | 0.03 | 0.06 | 0.25 | |
| pKU412 | pKU404 ΔampC | 4 | 2 | 0.25 | 0.125 | 0.06 | 0.125 | 0.5 | 0.03 | 0.06 | 0.25 | |
| pKU413 | pKU405 ΔampC | 8 | 4 | 0.5 | 0.125 | 0.125 | 0.125 | 1 | 0.03 | 0.06 | 0.25 | |
| pKU415 | pKU406 ΔampC | 8 | 4 | 0.25 | 0.125 | 0.125 | 0.125 | 0.5 | 0.03 | 0.06 | 0.25 | |
| pMW218 | Vector | 8 | 4 | 0.25 | 0.06 | 0.06 | 0.125 | 0.5 | 0.03 | 0.06 | 0.25 | |
| 8 | 2 | 0.25 | 0.125 | 0.06 | 0.125 | 0.5 | 0.03 | 0.06 | 0.25 | |||
| ML4953 (AmpD mutant) | pKU403 | Wild type | >128 | >128 | 128 | 2 | 0.5 | 0.5 | 32 | 0.03 | 0.25 | 1 |
| pKU404 | H-mut | >128 | >128 | >128 | 8 | 32 | 32 | >128 | 0.25 | 16 | 1 | |
| pKU405 | L-mut | >128 | >128 | >128 | 4 | 16 | 16 | >128 | 0.125 | 8 | 1 | |
| pKU406 | I-mut | >128 | >128 | >128 | 4 | 8 | 8 | >128 | 0.06 | 8 | 1 | |
| pKU407 | I-mut | >128 | >128 | >128 | 4 | 8 | 8 | >128 | 0.06 | 8 | 2 | |
| pKU408 | pKU403 ΔampR | 8 | 8 | 1 | 0.125 | 0.125 | 0.125 | 2 | 0.03 | 0.06 | 1 | |
| pKU409 | pKU404 ΔampR | 8 | 16 | 1 | 0.125 | 0.125 | 0.125 | 2 | 0.03 | 0.125 | 1 | |
| pKU410 | pKU405 ΔampR | 8 | 8 | 0.5 | 0.125 | 0.125 | 0.25 | 2 | 0.03 | 0.125 | 1 | |
| pKU414 | pKU406 ΔampR | 8 | 8 | 1 | 0.125 | 0.125 | 0.25 | 2 | 0.03 | 0.06 | 1 | |
| pKU411 | pKU403 ΔampC | 8 | 4 | 0.25 | 0.125 | 0.06 | 0.125 | 1 | 0.03 | 0.06 | 1 | |
| pKU412 | pKU404 ΔampC | 8 | 8 | 0.5 | 0.125 | 0.06 | 0.125 | 1 | 0.03 | 0.06 | 1 | |
| pKU413 | pKU405 ΔampC | 8 | 8 | 0.25 | 0.125 | 0.06 | 0.125 | 1 | 0.03 | 0.03 | 1 | |
| pKU415 | pKU406 ΔampC | 8 | 4 | 0.5 | 0.125 | 0.06 | 0.125 | 1 | 0.03 | 0.03 | 1 | |
| pMW218 | Vector | 4 | 4 | 0.25 | 0.125 | 0.06 | 0.125 | 1 | 0.03 | 0.06 | 1 | |
| 4 | 4 | 0.25 | 0.125 | 0.06 | 0.25 | 1 | 0.03 | 0.06 | 1 | |||
a
L-, I-, and H-mut, mutant plasmids with low, intermediate, and high levels of β-lactamase activity, respectively.
b
ABPC, ampicillin; CER, cephaloridine; CTM, cefotiam; LMOX, latamoxef; CTX, cefotaxime; CAZ, ceftazidime; CPDX, cefpodoxime; CFPM, cefepime; AZT, aztreonam; IPM, imipenem.