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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Cancer Res. 2019 May 22;79(14):3608–3621. doi: 10.1158/0008-5472.CAN-18-4055

Fig. 2. BC-derived, EV-encapsulated miR-9–5p, miR-203a-3p, and miR-195–5p are induced by chemotherapy and required for CSC regulation.

Fig. 2.

(A,B) Total RNA was extracted from an equal amount of EVs (A) or cell lysates (B) of PBS- or chemotherapy-treated MCF10A, MDA231, MCF-7, and BT474 cells. RT-qPCR was performed to determine levels of miR-9–5p, miR-203a-3p, and miR-195–5p using a cel-miR-39–3p spike-in control (for EV miRNA levels) or U6 internal control (for intracellular miRNA levels) for normalization. (C,D) MDA231, MCF-7, and BT474 cells were transfected with anti-miR-9–5p, anti-miR-203a-3p, anti-miR-195–5p oligonucleotides or mismatch control (25 pmol per 2×105 cells), and treated with EVs from the indicated producer cells for 48 h, before being analyzed by Western blots. *P<0.05, **P<0.01, ***P<0.001. Numbers below Western images indicate quantification after normalization to GAPDH with the first lane set as 1.