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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Cell Rep. 2022 Mar 15;38(11):110524. doi: 10.1016/j.celrep.2022.110524

Figure 3. Zbtb11Δ and Zfp131Δ knockout cells differentiate into multiple lineages.

Figure 3.

(A) Uniform manifold approximation and projection (UMAP) plot of Zbtb11Δ and H2b:Gfp (internal control) cells single-cell RNA-seq. Knockout cells were grown in −2i + LIF media (n = 1).

(B) Violin plots of genes expressed in each cluster in Zbtb11Δ and H2b:Gfp cells. ESC, mesoderm, endoderm, and ectoderm markers are expressed in distinct clusters.

(C) Expression of genes associated with pluripotency and germ layers in Zbtb11Δ cells. Zbtb11Δ cells segregate in different clusters compared with H2b::Gfp and express developmentally regulated genes.

(D) UMAP plot of Zfp131Δ and H2b:Gfp (internal control) cells single-cell RNA-seq. Knockout cells were grown in −2i + LIF media (n = 1).

(E) Violin plots of genes expressed in each cluster in Zfp131Δ and H2b:Gfp cells. ESC, mesoderm, endoderm, and ectoderm markers are expressed in distinct clusters.

(F) Expression of genes associated with pluripotency and germ layers in Zfp131Δ and H2b:Gfp UMAP plots. Zfp131Δ cells segregate in different clusters compared with H2b::Gfp and express developmentally regulated genes.

See also Figure S8.