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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Cell Rep. 2022 Mar 15;38(11):110524. doi: 10.1016/j.celrep.2022.110524

Figure 6. ZBTB11- and ZFP131-associated pro-differentiation genes gained H3K4me3 and lost NELF at TSSs and are transcribed in Zbtb11Δ and Zfp131Δ cells.

Figure 6.

(A–C) Metagene plots for (A) H3K4me3 signal distribution, (B) NELF signal distribution, and (C) H3K27me3 signal distribution at ZBTB11-associated genes in Zbtb11Δ versus WT (n = 2), at ZFP131 associated genes in Zfp131Δ versus WT (n = 2), and at co-occupied genes in Zbtb11Δ and Zfp131Δ versus WT. All comparisons were done in −2i + LIF. TTS, transcription termination site.

(D and E) Violin plots for Z score mRNA expression of genes that are (D) ZBTB11 bound along with NELF and H3K4me3 increase in Zbtb11 knockout (n = 1,191) and ZBTB11 and ZFP131 bound along with NELF and H3K4me3 increase in Zbtb11 knockout (n = 216) and (E) ZFP131 bound along with NELF and H3K4me3 increase in Zfp131 knockout (n = 915) and ZBTB11 and ZFP131 bound along with NELF and H3K4me3 increase in Zfp131 knockout (n = 216).

(F and G) GO enrichment analysis of significantly upregulated genes (F) with ZBTB11 binding and H3K4me3 increase (n = 531) and (G) with ZFP131 binding and H3K4me3 increase (n = 264) in +2i + LIF conditions.