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. 2022 Mar 18;13:868893. doi: 10.3389/fendo.2022.868893

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Copyright © 2022 Song, Pan, Chen and Jin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of NPVs. (A) Representative cryo-TEM images of NPVs. (Scale bar; 1 bar represents 200 nm) (B) Size distribution of NPVs obtained using DLS, NPVs are on average about 68 nm wide. (C) The expression of CD41 on NPVs was detected by western blot, and TSG101 was used as an internal reference. (D) ELISA detection of TGF-β content in platelets and NPVs. (E) Immunostaining of M1 macrophages after treatment with phosphate buffered saline (PBS) or NPVs. (Nuclei, blue; TGF-β, green) Scale bar = 100 μm, n = 3 per group.