Segmentation of iPSC-CMs, at medium seeding density, for the three imaging modalities, SYTO 24 fluorescence, DPC, and FPM. Insets for the fluorescence and DPC images show zoomed-in regions with segmentation overlays from Columbus (cyan inner: nucleus; outer: cell). The inset for the FPM image shows a zoomed-in region with overlay from QPI segmentation. All larger image scale bars represent 100 µm. All inset scale bars represent 20 µm.