Fig. 7.
Numerical simulations of the lateral shift behavior of the wavefront engineered PSF. (a) and (b) Intensity distributions of the PSF reconstructed on the Hz plane (top panel) for the movement of a point source along the z axis with the constant initial phase values (ci = 0) (a) and the optimized phase values (b) for the design parameters drange = 20 µm, N = 16, δ = 75 mm with a fluorescence wavelength of 560 nm. The horizontal and vertical scale bars are 0.2 mm and 2 µm, respectively. The color scale of the top panels is normalized to the maximum intensity of (b). The bottom panels show the corresponding phase distribution of the multiplexed CGHs applied to fluorescence signals. The red-dashed circle indicates the pupil size of the objective lens. (c) Wrapped phase variation along the ξ axis on the magnified area (inset) indicated by the blue-dotted rectangle in (b). Blue squares correspond to the actual sampling points of the SLM pixels used in our study.