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RT-qPCR efficiency with dsRNA standards.
| Genome | Slope | Y-intercept | R 2 | Efficiency |
|---|---|---|---|---|
| CsTLV1 | 3.29 | 38.730 | 0.995 | 100.1 |
| CsTLV2 | 3.25 | 37.624 | 0.995 | 100.3 |
The threshold cycles for a log10 dilution series are used to assess efficiency relative to 100% theoretical efficiency for a slope of 3.32 based on 10 replicates.
