Figure 2.

Treatment with rapamycin for eight weeks is sufficient to prevent reduced PC survival. A, Midline vermis sagittal sections are stained with anti-calbindin antibody and show that PC loss is prevented after eight weeks of rapamycin treatment, even after treatment cessation for four weeks. B, Quantification of PC numbers. Two-way ANOVA, Bonferroni's post hoc testing. *p < 0.05, **p < 0.01, ***p < 0.001 ****, and p < 0.0001. ns, not significant; VEH, vehicle; RAPA, rapamycin. Scale bar: 100 µm. Data are reported as mean ± SEM.