Figure 3.

Impact of eight-week rapamycin treatment on PC function. A, B, Rapamycin treatment paradigm results in improved PC (A) intrinsic firing rates and (B) excitability in mutant mice. C–E, Cell properties during recordings show (C) decreased membrane input resistance in mutant mice, (D) membrane capacitance remained unaffected, and (E) a decreased membrane time constant in the mutants. Recordings were performed at 13 weeks of age (4 weeks after treatment was stopped at 9 weeks of age). Two-way ANOVA, Bonferroni's post hoc testing. *p < 0.05, **p < 0.01, ***p < 0.001 ****, and p < 0.0001. ns, not significant; VEH, vehicle; RAPA, rapamycin. Scale bar: 100 µm. Data are reported as mean ± SEM.