Figure 4.

Treatment with rapamycin for four weeks is sufficient to prevent the onset of social impairments. A, Four-week rapamycin treatment paradigm (denoted by red line). Behavioral assessment then begins four weeks after cessation of rapamycin treatment. Critical period denotes period to develop abnormal behaviors. B, C, Treatment with rapamycin for four weeks prevents the onset of social deficits in (B) three-chamber social approach, social novelty, and (C) social olfactory testing. D, No changes in behavioral flexibility were observed between genotypes in the water Y maze; four weeks of rapamycin treatment had no effect on performance on Rday1. E, Motor learning in the accelerating rotarod. F, Open field testing did not reveal any differences between locomotor function between any group. G, H, No significant differences were found in (G) center time in open field or (H) time in elevated plus maze open arms between any group. Two-way ANOVA, Bonferroni's post hoc testing. *p < 0.05, **p < 0.01, ***p < 0.001 ****, and p < 0.0001. ns, not significant; VEH, vehicle; RAPA, rapamycin. Data are reported as mean ± SEM.