Figure 6.

Impact of four-week rapamycin treatment on PC function. A, B, Four weeks of rapamycin treatment prevented deficits in (A) PC intrinsic firing rates and (B) excitability. C–E, Cell properties during recordings revealed no significant differences in (C) membrane input resistance, (D) membrane capacitance, or (E) membrane time constant. Recordings were performed at nine weeks of age (4 weeks after treatment was stopped at 5 weeks of age). Two-way ANOVA, Bonferroni's post hoc testing. *p < 0.05, **p < 0.01, ***p < 0.001 ****, and p < 0.0001. ns, not significant; VEH, vehicle; RAPA, rapamycin. Scale bar: 100 µm. Data are reported as mean ± SEM.