Table 2.
Statistics for P7–P35 cohort
| Test/experiment | ||
|---|---|---|
| Figure 4, behavior characterization | ||
| Social approach | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.1321 | F(3,90) = 1.920 |
| Preference | p < 0.0001 | F(1,90) = 62.64 |
| Genotype | p = 0.1111 | F(3,90) = 2.060 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Novel animal, novel object | ||
| Control VEH n = 15 | p < 0.0001 | |
| Mutant VEH n = 10 | p = 0.4167 | |
| Control RAPA n = 13 | p = 0.0001 | |
| Mutant RAPA n = 14 | p < 0.0001 | |
| Social novelty | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.2467 | F(3,96) = 1.403 |
| Preference | p < 0.0001 | F(1,96) = 27.22 |
| Genotype | p = 0.5971 | F(3,96) = 0.6305 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Novel animal, novel object | ||
| Control VEH n = 17 | p = 0.0497 | |
| Mutant VEH n = 12 | p > 0.9959 | |
| Control RAPA n = 17 | p = 0.0002 | |
| Mutant RAPA n = 14 | p = 0.0380 | |
| Social olfaction | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p < 0.0001 | F(42,1050) = 4.653 |
| Scent | p < 0.0001 | F(14,1050) = 191.2 |
| Group | p < 0.0001 | F(3,1050) = 46.70 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Water | ||
| Control VEH n = 16 vs mutant VEH n = 16 | p > 0.9999 | |
| Control RAPA n = 21 vs mutant RAPA n = 21 | p > 0.9999 | |
| Control VEH n = 16 vs control RAPA n = 21 | p > 0.9999 | |
| Mutant VEH n = 16 vs mutant RAPA n = 21 | p > 0.9999 | |
| Almond | ||
| Control VEH n = 16 vs mutant VEH n = 16 | p = 0.4580 | |
| Control RAPA n = 21 vs mutant RAPA n = 21 | p > 0.9999 | |
| Control VEH n = 16 vs control RAPA n = 21 | p > 0.9999 | |
| Mutant VEH n = 16 vs mutant RAPA n = 21 | p = 0.6805 | |
| Social scent | ||
| Control VEH n = 16 vs mutant VEH n = 16 | p < 0.0001 | |
| Control RAPA n = 21 vs mutant RAPA n = 21 | p < 0.0001 | |
| Control VEH n = 16 vs control RAPA n = 21 | p > 0.9999 | |
| Mutant VEH n = 16 vs mutant RAPA n = 21 | p = 0.0003 | |
| Water Y maze | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p < 0.0001 | F(15,474) = 4.925 |
| Day | p < 0.0001 | F(15,474) = 120.6 |
| Group | p < 0.0001 | F(15,474) = 16.75 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Trial 1 | ||
| Control VEH n = 15 vs mutant VEH n = 17 | p > 0.9999 | |
| Control RAPA n = 26 vs mutant RAPA n = 25 | p > 0.9999 | |
| Control VEH n = 15 vs control RAPA n = 26 | p > 0.9999 | |
| Mutant VEH n = 17 vs mutant RAPA n = 25 | p > 0.9999 | |
| Trial 2 | ||
| Control VEH n = 15 vs mutant VEH n = 17 | p > 0.9999 | |
| Control RAPA n = 26 vs mutant RAPA n = 25 | p > 0.9999 | |
| Control VEH n = 15 vs control RAPA n = 26 | p > 0.9999 | |
| Mutant VEH n = 17 vs mutant RAPA n = 25 | p > 0.9999 | |
| Trial 3 | ||
| Control VEH n = 15 vs mutant VEH n = 17 | p > 0.9999 | |
| Control RAPA n = 26 vs mutant RAPA n = 25 | p > 0.9999 | |
| Control VEH n = 15 vs control RAPA n = 26 | p > 0.9999 | |
| Mutant VEH n = 17 vs mutant RAPA n = 25 | p > 0.9999 | |
| Rev. trial 1 | ||
| Control VEH n = 15 vs mutant VEH n = 17 | p < 0.0001 | |
| Control RAPA n = 26 vs mutant RAPA n = 25 | p = 0.0041 | |
| Control VEH n = 15 vs control RAPA n = 26 | p = 0.3740 | |
| Mutant VEH n = 17 vs mutant RAPA n = 25 | p > 0.9999 | |
| Rev. trial 2 | ||
| Control VEH n = 15 vs mutant VEH n = 17 | p < 0.0001 | |
| Control RAPA n = 26 vs mutant RAPA n = 25 | p = 0.2559 | |
| Control VEH n = 15 vs control RAPA n = 26 | p > 0.9999 | |
| Mutant VEH n = 17 vs mutant RAPA n = 25 | p = 0.0006 | |
| Rev. trial 3 | ||
| Control VEH n = 15 vs mutant VEH n = 17 | p < 0.0001 | |
| Control RAPA n = 26 vs mutant RAPA n = 25 | p > 0.9999 | |
| Control VEH n = 15 vs control RAPA n = 26 | p > 0.9999 | |
| Mutant VEH n = 17 vs mutant RAPA n = 25 | p < 0.0001 | |
| Rotarod | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.9952 | F(12,235) = 0.2504 |
| Day | p < 0.0001 | F(4,235) = 12.38 |
| Group | p < 0.0001 | F(3,235) = 14.26 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Day 1 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p = 0.6610 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.2247 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| Day 2 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p = 0.0119 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.0254 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| Day 3 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p = 0.0294 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.0626 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| Day 4 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p = 0.0748 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.1765 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| Day 5 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p = 0.0073 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.3565 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p = 0.1602 | |
| Open field | ||
| Two-way ANOVA | ||
| Distance moved | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.9845 | F(42,705) = 0.5823 |
| Minute | p = 0.5856 | F(14,705) = 0.8758 |
| Group | p < 0.0001 | F(3,705) = 9.465 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| 0:00–1:00 (60 seconds) | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p = 0.7247 | |
| Control VEH n = 12 vs control RAPA n = 13 | p > 0.9999 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 1:00–2:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p = 0.3628 | |
| Control VEH n = 12 vs control RAPA n = 13 | p > 0.9999 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p = 0.2082 | |
| 2:00–3:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p = 0.4648 | |
| Control VEH n = 12 vs control RAPA n = 13 | p > 0.9999 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 3:00–4:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p > 0.9999 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 4:00–5:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p = 0.2421 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.5267 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 5:00–6:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p > 0.9999 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 6:00-7:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p > 0.9999 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 7:00–8:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p > 0.9999 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 8:00–9:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p = 0.2996 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.2294 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 9:00–10:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.7791 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p = 0.0953 | |
| 10:00–11:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p > 0.9999 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 11:00–12:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p > 0.9999 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 12:00–13:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p > 0.9999 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 13:00–14:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p = 0.8308 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.0227 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| 14:00–15:00 | ||
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.5178 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p > 0.9999 | |
| Time in center | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.7432 | F(1,47) = 0.1086 |
| Treatment | p = 0.1829 | F(1,47) = 1.827 |
| Genotype | p = 0.4546 | F(1,47) = 0.5684 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Control VEH n = 12 vs mutant VEH n = 10 | p > 0.9999 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p = 0.8279 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.9436 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p = 0.4841 | |
| Elevated plus maze | ||
| Two-way ANOVA | ||
| Open arms | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.1322 | F(1,47) = 2.347 |
| Treatment | p = 0.6031 | F(1,47) = 0.2741 |
| Genotype | p = 0.4877 | F(1,47) = 0.4892 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Control VEH n = 12 vs mutant VEH n = 10 | p = 0.2909 | |
| Control RAPA n = 13 vs mutant RAPA n = 16 | p > 0.9999 | |
| Control VEH n = 12 vs control RAPA n = 13 | p = 0.3207 | |
| Mutant VEH n = 10 vs mutant RAPA n = 16 | p = 0.9617 | |
| Figure 5, cell survival | ||
| Cell count | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.0006 | F(1,10) = 24.22 |
| Treatment | p = 0.0015 | F(1,10) = 18.56 |
| Genotype | p < 0.0001 | F(1,10) = 41.95 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Control VEH n = 4 vs mutant VEH n = 3 | p < 0.0001 | |
| Control RAPA n = 4 vs mutant RAPA n = 3 | p = 0.5947 | |
| Control VEH n = 4 vs control RAPA n = 4 | p > 0.9999 | |
| Mutant VEH n = 3 vs mutant RAPA n = 3 | p = 0.0002 | |
| Mutant VEH n = 3 vs control RAPA n = 3 | p = 0.0001 | |
| Figure 6, physiology | ||
| PC intrinsic firing rate | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.0100 | F(1,113) = 6.870 |
| Treatment | p = 0.0173 | F(1,113) = 5.832 |
| Genotype | p = 0.0110 | F(1,113) = 6.681 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Control VEH n = 43 vs mutant VEH n = 16 | p = 0.0008 | |
| Control RAPA n = 41 vs mutant RAPA n = 17 | p > 0.9999 | |
| Control VEH n = 43 vs control RAPA n = 41 | p > 0.9999 | |
| Mutant VEH n = 16 vs mutant RAPA n = 17 | p = 0.0072 | |
| Mutant VEH n = 16 vs control RAPA n = 41 | p = 0.0042 | |
| PC evoked responses | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.2388 | F(42,1344) = 1.149 |
| Current step | p < 0.0001 | F(14,1344) = 564.4 |
| Group | p < 0.0001 | F(3,1344) = 99.38 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Control VEH n = 39 vs mutant VEH n = 17 | p < 0.0001 | |
| Control RAPA n = 26 vs mutant RAPA n = 17 | p > 0.9999 | |
| Control VEH n = 39 vs control RAPA n = 26 | p = 0.0326 | |
| Mutant VEH n = 17 vs mutant RAPA n = 17 | p = 0.0070 | |
| Mutant VEH n = 17 vs control RAPA n = 26 | p < 0.0001 | |
| Membrane input resistance | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.4496 | F(1,71) = 0.5780 |
| Treatment | p = 0.9045 | F(1,71) = 0.01451 |
| Genotype | p = 0.0316 | F(1,71) = 4.808 |
| Bonferroni's multiple comparisons test | Adjusted p value | |
| Control VEH n = 16 vs mutant VEH n = 15 | p = 0.1140 | |
| Control RAPA n = 25 vs mutant RAPA n = 19 | p = 0.5429 | |
| Control VEH n = 16 vs control RAPA n = 25 | p > 0.9999 | |
| Mutant VEH n = 15 vs mutant RAPA n = 19 | p > 0.9999 | |
| Mutant VEH n = 15 vs control RAPA n = 25 | p = 0.5985 | |
| Membrane capacitance | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.4741 | F(1,62) = 0.5186 |
| Treatment | p = 0.2037 | F(1,62) = 1.650 |
| Genotype | p = 0.1059 | F(1,62) = 2.692 |
| Membrane time constant | ||
| Two-way ANOVA | ||
| ANOVA table | p value | F (DFn,DFd) |
| Interaction | p = 0.2520 | F(1,62) = 1.337 |
| Treatment | p = 0.2431 | F(1,62) = 1.389 |
| Genotype | p = 0.2269 | F(1,62) = 1.490 |
| Mouse age at time of testing (all experiments) | ||
| 4-week treatment paradigm | ||
| Rotarod | 9 weeks | Rotarod |
| Open field | 10 weeks | Open field |
| Elevated plus maze | 10 weeks | Elevated plus maze |
| Social interaction (three-chamber) | 11 weeks | Social interaction (three-chamber) |
| Social olfaction | 11 weeks | Social olfaction |
| Water Y maze | 12 weeks | Water Y maze |
| Immunohistochemistry | 9–12 weeks | Immunohistochemistry |
| Acute slice physiology | 9–12 weeks | Acute slice physiology |