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. 2022 Mar 30;19(1):468–480. doi: 10.1080/15476286.2022.2057725

Figure 3.

Figure 3.

A bulge of 1 nt is required for 3’ end Y RNA cleavage. (A) All L2a loop mutants except for L2a Δ8 nt and L2a Δ9 nt were predicted to fold similarly to wild type RNY5. (B) Sequential deletion of internal loop L2a had a small effect on ysRNA production from either the 3’ or the 5’ end of RNY5. Except for L2a loop Δ3 nt to L2a loop Δ6 nt, mutants showed decreased ysRNA levels when compared to wild type RNY5 derived ysRNAs. L2a loop Δ9 nt did not produce ysRNAs from neither the 3’ nor the 5’ end of RNY5. (C) Most of the RNY5 substitution mutants except for M9 and M14 folded similarly to wild type RNY5. (D) Only M5 and M7 were less efficiently processed to ysRNAs when compared to wild type RNY5.