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. 2022 Mar 30;19(1):468–480. doi: 10.1080/15476286.2022.2057725

Figure 5.

Figure 5.

Conserved UUAU motif involved in 3’ and 5’ end cleavage. (A) Wild type RNY5 structure with the structural feature loop L2b. The structural feature loop L2b at positions 23–26 is shown in Orange. (B) Predicted structures of RNY5 L1 mutants with high or no ysRNA accumulation. RNY5 L1 mutants that produce ysRNAs in a good amount are shown in the upper panel whereas the RNY5 L1 mutants with no ysRNAs from the 3’ and 5’ end are shown in the lower panel. (C) RNY5 L1 mutants with an intact internal loop L2b generate a good amount of ysRNAs whereas RNY5 mutants with a shorter internal loop L2b produce less/no ysRNA from the 3’ nor the 5’ end of RNY5. (D) Predicted structures with mutated loop L2b with the same structure than wild type RNY5 (upper panel) or different structure than wild type RNY5. (E) Mutations in loop L2b resulted in a lower accumulation of ysRNAs from the 3’ and 5’ end of RNY5.