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. 2022 Mar 31;11(1):2057837. doi: 10.1080/2162402X.2022.2057837

Figure 3.

Figure 3.

Myeloma-derived IL-32γ promotes PD-L1 expression in macrophages through PR3. (a) IL-32γ mRNA expression was quantified by qRT-PCR in monocyte-induced macrophages and MM cell lines. (b) Protein expression of IL-32 in monocyte-induced macrophages and MM cell lines was measured using western blotting. (C-D, F-G) Detection of IL-32 expression in IL-32γ-overexpressing MM cells (H929 and JJN3) by qRT-PCR (c and f) and western blotting (d and g). (e, h) Exosomes from IL-32γ-overexpressing H929 (e) and JJN3 cells (h) were incubated with macrophages, the expression of PD-L1 was examined by flow cytometry. (i) Macrophages were transfected with control siRNA or PR3 siRNA and then treated with or without IL-32γ (40 ng/ml) for 24 h; the levels of PD-L1 expression were determined by flow cytometry. (j) Western blotting was performed to measure the expression of PFKFB3 and PD-L1 when macrophages were transfected with control siRNA or PR3 siRNA and then treated with IL-32γ (40 ng/ml) for 24 h; Data are presented as the mean ± SD of at least three independent experiments; *p < .05, **p < .01, ***p < .001, #, not significant.