Extended Data Figure 7 |. LC10a signaling is necessary and amplified during courtship.

a, Schematic of LC10a neurons expressing GtACR1 with approximate ROIs used for silencing (or sham-silencing) in a single hemisphere. b, Average turning of one male to the visual stimulus during silencing of LC10a neurons in the right hemisphere versus sham trials. Note that male fails to execute turns in the direction ipsilateral to silencing. c, Average turning in the direction ipsilateral and contralateral to the hemisphere where LC10a was silenced, compared to sham trials. d, Image of LC10a axon terminals expressing jGCaMP7f in the anterior optic tubercle. e, Example of functional response (ΔF/F₀) of LC10a neurons expressing jGCaMP7f during a courtship. Note that, in difference from recordings made using GCaMP6s (Fig. 2b), calcium transients return to baseline in-between responses with this faster indicator. e, Average evoked LC10a responses (ΔF/F₀) to one stimulus cycle for animals expressing GCaMP6s versus jGCaMP7f. f, Average change in LC10a gain (distance between peak and through of evoked responses) for animals expressing GCaMP6s versus jGCaMP7f. g, Example of LC10a functional responses during courtship versus during a later period of undirected running with similar linear speed. All shaded line plots are mean±s.e.m.; n.s. indicates p > 0.05, and **indicates p < 0.01. Details of statistical analyses and group sizes are given in Supplementary Table 1.