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. 2022 Mar 30;42(13):2631–2646. doi: 10.1523/JNEUROSCI.1459-21.2022

Figure 10.

Figure 10.

Extracellular GPI promotes neuronal dendritic development without affecting glycolysis. A, Representative images of primary neurons dissected from WT brains. The neurons treated with or without GPI in different concentrations as indicated from DIV4 to DIV14, and stained with MAP2 antibodies at DIV14. Scale bars: 20 μm. B, C, Quantitative analysis of the total basal dendritic length (B, one-way ANOVA, F(2,6) = 0.9092; PBS vs GPI 100 ng, *p = 0.01; PBS vs GPI 300 ng, **p = 0.0047) and the basal dendritic complexity (C, univariate ANOVA, PBS vs GPI 100 ng, F(1,4) = 11.258, *p = 0.028; PBS vs GPI 300 ng, F(1,4) = 21.435, **p = 0.01) of cultured neurons in A. PBS, 100 neurons; GPI 100 ng, 85 neurons; GPI 300 ng, 91 neurons; n = 3 independent experiments. D, E, Representative profile of glycolysis stress assay (D) showing the ECAR of the cultured neurons treated with or without GPI (100 ng). Graph in E showing glycolysis, glycolytic capacity, glycolytic reserve, and nonglycolytic acidification of neurons in D. Data are presented as mean ± SEM; n.s., no significance, p > 0.05, *p < 0.05, **p < 0.01.