Table 1. PCR primers, annealing temperatures, and amplicon size used to detect the putative virulence genes in the isolates.

Primers	Sequences (5’–3’)	Tm (°C)	Amplicon size (bp)	References
Ace	Ace-F: CAGGCCAACATCAAGCAACA	65	125	Al-Talib et al. (2015)
	Ace-R: GCTTGCCTCGCCTTCTACAA
Agg	Agg-F: AAGAAAAAGAAGTAGACCAAC	53	1553	Espeche et al. (2012)
	Agg-R: AAACGGCAAGACAAGTAAATA
Asa1	Asa1-F: GCACGCTATTACGAACTATGA	56	375	Vankerckhoven et al. (2004)
	Asa-R: TAAGAAAGAACATCACCACGA
Cpd	Cpd-F: TGGTGGGTTATTTTTCAATTC	50	782	Eaton & Gasson (2001)
	Cpd-R: TACGGCTCTGGCTTACTA
CylA	CylA-F: ACTCGGGGATTGATAGGC	60	688	Vankerckhoven et al. (2004)
	CylA-R: GCTGCTAAAGCTGCGCTT
ClyB	ClyB-F: ATTCCTACCTATGTTCTGTTA	56	843	Vankerckhoven et al. (2004)
	ClyB-R: AATAAACTCTTCTTTTCCAAC
EfaAfs	EfaAfs-F: GACAGACCCTCACGAATA	56	705	Eaton & Gasson (2001)
	EfaAfs-R: AGTTCATCATGCTGTAGTA
GelE	GelE-F: CGAAGTTGGAAAAGGAGGC	50	372	Al-Talib et al. (2015)
	GelE-R: GGTGAAGAAGTTACTCTGA