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. 2022 Mar 18;9:870680. doi: 10.3389/fnut.2022.870680

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Copyright © 2022 Liu, Gu, Qu, Bao, Li and Cai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Total bile acids (TBA) content and the expression of genes involved in cholesterol transformation were elevated in livers of PEDV-infected piglets. (A) TBA contents in the livers of piglets were analyzed and normalized to total protein concentrations. (B,C) Western blotting analysis was performed to evaluate the expression of Cholesterol-7a-hydroxylase (CYP7A1) and cholesterol-27a-hydroxylase (CYP27A1) at the protein level. Based on the intensity of protein bands, the relative expression levels of CYP7A1 and CYP27A1 were determined by normalization to GAPDH. The data are shown as the means ± SEM, n = 7, *P < 0.05, **P < 0.01, using the two-tailed Student t-test.