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. 2022 Jan 14;13(2):2028–2043. doi: 10.1080/21655979.2021.2019872

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — ADIRF-AS1 directly sponges miR-761 in OS. (a) The localization of ADIRF-AS1 in OS cells was demonstrated by subcellular fractionation experiments. (b) The putative targets of ADIRF-AS1 were searched using ENCORI and miRDB. (c) After ADIRF-AS1 knockdown in OS cells, the levels of miR-214–3p, miR-514a-5p, miR-3619–5p, miR-761, and miR-1913 were quantified by qRT-PCR. (d) Schematic representation of the wt and mut binding sequences of miR-761 within ADIRF-AS1. (e) The luciferase activity of wt-ADIRF-AS1 or mut-ADIRF-AS1 was detected after treatment with the miR-761 mimic or NC mimic. (f) The expression levels of miR-761 in OS tissues was determined by qRT-PCR. (g) Pearson’s correlation coefficient analysis was used to test the relationship between ADIRF-AS1 and miR-761 expression in OS tissues. (h) A RIP assay showed that ADIRF-AS1 is present with miR-761 in Ago2-containing immunoprecipitated RNA in OS cells. **P < 0.01.