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IRS1 overexpression abrogates the inhibitory activity of si-ADIRF-AS1 on OS cells. (a) The efficiency of pcDNA3.1-IRS1 transfection was validated by Western blotting. (b, c) Proliferation and apoptosis were detected in OS cells after si-ADIRF-AS1 and pcDNA3.1-IRS1 or pcDNA3.1 cotransfection. (d, e) The migratory and invasive capacities were examined in treated OS cells as described above. *P < 0.05 and **P < 0.01.
