Figure 1.

Huaier constrains LC angiogenesis and tumor growth in vivo and in vitro. (a) CCK-8 detection of cell proliferation; (b) Flow cytometry detection of cell apoptosis; (c) Transwell detection of cell migration and invasion; (d) Matrigel tube formation test of the angiogenesis ability (200 ×); (e) Tumor volume and mass (n = 6); (f) Immunohistochemical characterization of CD31 protein and calculation of angiogenesis (n = 6); Expression of the values was as mean ± SD (N = 3 in cell experiments). In the Control and the Huaier groups. * vs. the Control, P < 0.05. Attached Figure 1 Down-regulation of let-7 family expression is presented in LC A. qRT-PCR to detect let-7 family expression level in LC; B. qRT-PCR to detect let-7d-5p expression levels in immortalized human lung cell line Beas-2B and LC cell lines (A549, H460, H1299, H1650 and H1975). The values were expressed as mean ± SD (cell experiments were repeated 3 times independently). One-way ANOVA was applied to calculate the significance of each group. The variance was corrected by Tukey test. * vs. the Normal group, P < 0.05. 0.05.