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. 2022 Mar 1;13(3):6698–6710. doi: 10.1080/21655979.2021.2012619

Figure 2.

Figure 2.

Restrained let-7d-5p is in LC; elevated let-7d-5p accelerates Huaier’s therapy on LC. (a) RT-qPCR detection of let-7d-5p in clinical samples; (b) RT-qPCR verification of the successful transfection; (c) CCK-8 detection of cell proliferation; (d) Flow cytometry detection of cell apoptosis; (e) Transwell detection of cell migration and invasion; (f) Matrigel tube formation test of the angiogenesis ability (200 ×); (C–F) after elevated let-7d-5p. Expression of the values was as mean ± SD (N = 3). * vs. the Huaier + mimic NC, P < 0.05. Attached Figure 2 Huaier represses the proliferation, migration, invasion and angiogenesis of H460 cells, but promotes apoptosis. (a) CCK-8 to detect the proliferation of cells in the Control and the Huaier groups; (B)Cell apoptosis in the Control and the Huaier groups detected by flow cytometry; (c) Transwell to detect the migration and invasion of cells in the Control and the Huaier groups; (d) Matrigel tube formation assay to detect angiogenesis in the Control and the Huaier groups (200 ×); The values were expressed as mean ± SD (cell experiments were repeated 3 times independently). One-way ANOVA was applied to calculate the significance of each group. The variance was corrected by Tukey test. * vs. the Control group, P < 0.05.