Figure 3.

Ubiquitin-specific peptidase 2 overexpression suppressed clear cell renal cell carcinoma cells proliferation in vitro and in vivo. (a) The over-expression efficiency of ubiquitin-specific peptidase 2 in clear cell renal cell carcinoma cells shown using quantitative real-time polymerase chain reaction. (b, c) The results of Cell Counting Kit-8 and clone formation assays showed that ubiquitin-specific peptidase 2 overexpression suppressed the proliferation rate of clear cell renal cell carcinoma cells. (d) Wound healing assays demonstrated that ubiquitin-specific peptidase 2 overexpression inhibited migration ability of clear cell renal cell carcinoma cells. Both Vector and stably overexpressing ubiquitin-specific peptidase 2 groups for 786–0 cells healed at 48 hours. (e) Invasion assays revealed that ubiquitin-specific peptidase 2 overexpression prevented the invasive ability of clear cell renal cell carcinoma cells. (f) The size of tumor was significantly inhibited in the stably overexpressing ubiquitin-specific peptidase 2 vector group than in the lentivirus vector group. (g) Tumors in ubiquitin-specific peptidase 2 overexpression group were relatively lighter in weight. (h) Tumor growth curves for the lentivirus-Vector group and lentivirus-overexpression-ubiquitin-specific peptidase 2 group. Cells in the Vector and overexpression-ubiquitin-specific peptidase 2 groups were transfected with plasmid. The lentivirus-Vector and lentivirus-overexpression- ubiquitin-specific peptidase 2 groups were transduced with lentivirus. **p < 0.01 and, ***p < 0.001.