Skip to main content
. 2022 Feb 13;13(2):2552–2565. doi: 10.1080/21655979.2021.2018538

Figure 4.

Figure 4.

Effect of LINC00525 on hypoxic glycolysis is HIF-1α dependent.

(A) 24 hours after being cultured under normoxia or hypoxia, the enzymatic activity of LDHA in SW620, SW1116 and LoVo cells were measured and compared. Data shown are mean ±SD (n = 3). *** P < 0.001 between two groups.(B) 24 hours after being cultured under normoxia or hypoxia, and then analyzed GLUT1 expression using immunofluorescence.(C) LoVo cells expressing either si-NC or si-525 were transfected with or without Flag-HIF-1α, as indicated. 24 hours later, LoVo cells were cultured under normoxia or hypoxia for another 24 hours, and then being harvested to detect HIF-1α expression using immunoblotting.(D) LoVo cells expressing either si-NC or si-525 were transfected with or without Flag-HIF-1α, as indicated. 24 hours later, LoVo cells were cultured under normoxia or hypoxia for another 24 hours, we compared lactate levels in the culture medium of LoVo. Data shown are mean ±SD (n = 3). *** P < 0.001 between two groups.(E) LoVo cells expressing either si-NC or si-525 were transfected with or without Flag-HIF-1α, as indicated. 24 hours later, LoVo cells were cultured under normoxia or hypoxia for another 24 hours, we compared intracellular glucose levels of LoVo. Data shown are mean ±SD (n = 3). *** P < 0.001 between two groups.