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. 2022 Feb 13;13(2):2552–2565. doi: 10.1080/21655979.2021.2018538

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — LINC00525 promoted UBE2Q1 expression by sponging to miR-338-3p.

(A) Schematic diagram of binding sites of the 3’UTR of LINC00525 (up) or UBE2Q1 mRNA (down) and miR-338-3p.(B) LoVo cells expressing either si-NC or si-525 were transfected, as indicated. 24 hours later, LoVo cells were cultured under normoxia or hypoxia for another 24 hours, and then being harvested to detect miR-338-3p expression. Data shown are mean ±SD (n = 3). *** P < 0.001 between two groups.(C and D) LoVo cells expressing either miR-338-3p-nc (nc), miR-338-3p-mimic (mimic) or miR-338-3p-inhibitor (inhibitor), as indicated. 24 hours later, LoVo cells were cultured under normoxia or hypoxia for another 24 hours, and then being harvested to detect miR-338-3p (C) or LINC00525 (D) expression. Data shown are mean ±SD (n = 3). *** P < 0.001 between two groups.(E) LoVo cells expressing either miR-338-3p-nc (nc), miR-338-3p-mimic (mimic) or miR-338-3p-inhibitor (inhibitor) were transfected with LINC00525 wild type (WT) or mutant (MUT) transcript, and then detected luciferase activity. Data shown are mean ±SD (n = 3). *** P < 0.001 between two groups.(F) LoVo cells expressing either miR-338-3p-nc (nc), miR-338-3p-mimic (mimic) or miR-338-3p-inhibitor (inhibitor) were transfected with UBE2Q1 wild type (WT) or mutant (MUT) transcript, and then detected luciferase activity. Data shown are mean ±SD (n = 3). *** P < 0.001 between two groups.(G) LoVo cells expressing either si-NC, si-525, miR-338-3p-nc (nc) or miR-338-3p-mimic (mimic), as indicated. 24 hours later, LoVo cells were cultured under normoxia or hypoxia for another 24 hours, and then being harvested to detect UBE2Q1 protein expression. Data shown are mean ±SD (n = 3). *** P < 0.001 between two groups.