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. 2022 Feb 28;13(3):6332–6342. doi: 10.1080/21655979.2021.2005988

Figure 2.

Figure 2.

Synergistic inhibition impacts of Rhein and RAD001 on MGC-803 cell proliferation and invasion. A. structure of RAD001. B. MGC-803 cells were treated with 0, 2.5, 5, 10, 20, 40, 80, 160 nM RAD001. after 48 hours, IC50 value for RAD001 dose response was calculated using MTT. C. Rhein (80 μM) and RAD001 (40 nM) were used for MGC-803 cells, then cell viability was measured using MTT. D. Rhein (80 μM) and RAD001 (40 nM) were used for MGC-803 cells, then MGC-803 cell invasion was detected using transwell. E. EdU-staining was applied to detect MGC-803 cell proliferation. EdU-positive cells of MGC-803 cells after 80 μM Rhein or 40 nM RAD001 treatment for 48 h (×100). F. Light microscope images of MGC-803 cells were observed. G. protein levels of EMT-related molecules E-cadherin, N-cadherin, vimentin were measured using Western blotting. *p < 0.05.