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. 2022 Feb 13;13(3):4857–4868. doi: 10.1080/21655979.2022.2036400

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — miR-664b-3p specifically regulates the expression of BUB3 protein. (a) ENCORI and miRWalk predicted the target of the miR-664b-3p. (b) The relative expression level of mRNA of different mitogen-related proteins in cells transfected with miR-664b-3p mimics and NC mimics. **P < 0.01, ***P < 0.001 vs. NC mimic groups. (c)Specific regulation effect of miR-664b-3p on the expression of BUB3 was detected using luciferase assay. ***P < 0.001 vs. NC mimic groups. (d) Expression relation between miR-664b-3p and BUB3. ***P < 0.001 vs. NC inhibitor groups. (e) A high expression level was detected in COAD using TCGA. **P < 0.01 vs. Normal tissues. (f) The expression situation of BUB3 protein in CC cell lines, including HCT116, SW480, DLD-1, SW620, and SW116. **P < 0.01, ***P < 0.001 vs. NCM460 cell lines.