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. 2022 Feb 24;13(3):6208–6221. doi: 10.1080/21655979.2022.2037249

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Increasing miR-23a-3p expression was able to accelerate proliferation, invasion, as well as migration of cancer cells. (a) We applied CCK-8 assay for detection of cell viability in RBE cells after transfection with miR-23a-3p mimics or mimics NC. (b-c) Colony formation as well as EdU assays examined proliferation ability of cells when miR-23a-3p overexpression, respectively. (d) Transwell assays aimed to observed cell invasion as well as migration when miR-23a-3p overexpression. (e) Wound healing assay detected cell migration when miR-23a-3p overexpression. (f) Quantitation of proteins correlated to apoptosis (Bax and Bcl-2), EMT pathway biomarkers correlated to tumor transition (Vinmentin and E-cadherin) and cell cycle protein (CyclinD1) were examined by Western blot.