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. 2022 Feb 24;13(3):6208–6221. doi: 10.1080/21655979.2022.2037249

Figure 6.

Figure 6.

Exosome transmitted miR-23a-3p and motivated proliferation, invasion, as well as migration of cholangiocarcinoma cancer cells. (a) The morphology of exosomes was confirmed via electron microscopy. (b) The distribution of particle size was observed by NTA analysis. (c) Western blot detected exosome specific protein. (d) We labeled the exosomes extracted then cultured them with RBE cells. MiR-23a-3p level in RBE cells co-cultured with exosomes from HUCCT1 cells after transfection with miR-23a-3p mimics was greater than that in the mimics NC group. (e-f) After transfecting with mimics exosome or NC exosome in RBE cells, CCK-8 as well as colony formation assays aimed to confirm cell proliferation. (g) Transwell assays evaluated invasion as well as migration ability of transfected RBE cells.