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. 2022 Feb 3;13(2):4185–4200. doi: 10.1080/21655979.2022.2026727

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Identification of circPLK1 in NSCLC and its prognostic significance. (a) The exosomes derived from the serum of NSCLC patients and healthy subjects were analyzed using TEM. (b) The size of exosomes was evaluated by NTA analysis. (c) HSP70, CD63, CD81 and Galectin9 were detected using Western blotting in exosome samples.Total protein staining was shown as the loading control. (d) CircPLK1 expression in serum exosomes of NSCLC patients and healthy controls was quantified by qRT-PCR. (e) ROC curve was used to analyze the circPLK1 predictability. (f) CircPLK1 expression in NSCLC tissues and adjacent normal tissues. (g) The relative expression level of circPLK1 in a panel of NSCLC cell lines. (h) Kaplan Meier Curve and log-rank test were used to compare the overall survival in NSCLC patients. *P < 0.05; **P < 0.01; ***P < 0.001.