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. 2022 Mar 31;23:21. doi: 10.1186/s12868-022-00703-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — PE-conjugated antibody outperforms FITC and APC conjugated antibodies in detecting microglia LAG3. A Distribution of fluorescence intensities from FITC conjugated isotype control antibody labeled microglia with the previous blocking by mouse IgG, CD16/CD32/CD16.2 antibodies, or the combination of mouse IgG and CD16/CD32/CD16.2 antibodies. B Distribution of fluorescence intensities from nontreated microglia and isotype control antibody treated microglia. C Distribution of fluorescence intensities from nontreated microglia and FITC, PE, and APC conjugated LAG-3 antibodies and their respective isotype control antibodies treated microglia. D Median fluorescence intensity (MFI) of microglia as treated in (C). E Comparison of MFI _LAG-3/MFI _IgG of FITC, PE, and APC conjugated LAG-3 antibodies (n = 4 mice). Bars represent the means ± standard deviation (SD). Comparisons were made by one-way ANOVA test with Tukey’s post hoc multiple comparisons test. *P < 0.05; **P < 0.01; NS, not significantly different