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. 2022 Mar 31;23:21. doi: 10.1186/s12868-022-00703-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — PE-conjugated antibody outperforms FITC and APC conjugated antibodies in detecting microglia TIM-3. A Distribution of fluorescence intensities from nontreated microglia, FITC, PE, and APC conjugated TIM-3 antibodies treated, and their respective isotype control antibody-treated microglia. B The MFI of microglia treated as in (A). C Comparison of MFI _TIM-3/MFI _IgG of FITC, PE, and APC conjugated TIM-3 antibodies (n = 5 mice). Bars represent means ± SD. Comparisons were made by one-way ANOVA test with Tukey’s post hoc multiple comparisons test. *P < 0.05; **P < 0.01; NS, not significantly different