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. 2022 Feb 3;13(1):1049–1061. doi: 10.1080/21655979.2021.2017589

Figure 2.

Figure 2.

LINC00472 knockdown alleviates LPS-induced cardiac dysfunction in vitro. (a) The viability of AC-16 cardiomyocytes was measured by CCK-8 assay. (b and c) Levels of TNF-α (b) and IL-1β (c) in the supernatants were measured by ELISA. (d) LINC00472 expression in AC-16 cells from Control group and LPS group was detected by RT-qPCR. (e) RT-qPCR analysis was used to assess the transfection efficiency of LINC00472 knockdown. (f) CCK-8 assay was adopted to detect the viability of AC-16 cardiomyocytes from Control group, LPS group, LPS+si-NC group, or LPS+si-LINC00472 group, respectively. (g) Flow cytometry was performed to detect AC-16 cardiomyocyte in each group. (h and i) TNF-α (h) and IL-1β (i) expression levels in the supernatants from each group were detected by ELISA. All data were performed at least three independent experiments. Data were expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.