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. 2022 Apr 1;22:161. doi: 10.1186/s12870-022-03556-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig.6 — GmSPX8 positively regulated GmPTF1 and its downstream genes. A GmSPX8 regulated the transcriptional activity of GmPTF1. 1. PGreenII62-SK empty vector and E-box-LUC, 2. PSK-GmSPX8 and E-box-LUC, 3. PSK-GmPTF1 and E-box-LUC, 4. PSK-GmPTF1 and PSK-GmSPX8 and E-box-LUC. B Relative luciferase activity of tobacco leaves expressing the indicated constructs. C Effect of GmSPX8 on the transcription activity of GmPTF1 in yeast cells. D β-galactosidase activity of yeast cells expressing both GmSPX8 and GmPTF1 or GmPTF1 alone. Expression abundance of GmPTF1 (E), GmEXBL1 (F) and GmEXPB2 (G) in nodules of wild type and GmSPX8 overexpressed nodules under P-sufficient and P-deficient conditions