Figure 2.

Silencing of SP1 or CXCR4 ameliorates LPS-induced inflammatory response and apoptosis in H9c2 cells. (a) qRT-PCR and (b) Western blot analysis of CXCR4 expression in H9c2 cells transfected with si-CXCR4, pcDNA-CXCR4, or their matched controls. H9c2 cells were transfected with si-SP1, si-CXCR4, or their corresponding controls, and then treated with 10 μg/mL of LPS for 24 h. (c) Cell viability was examined using CCK-8 assay. (d) The release of LDH was examined using a commercial kit. (e) qRT-PCR and (f) ELISA assays were performed to determine the levels of TNF-α, IL-8, and IL-6 in H9c2 cells. (g) TUNEL staining was carried out to evaluate the apoptosis of H9c2 cells. (h) The activity of caspase-3 was measured to assess cell apoptosis. ***P < 0.001.