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. 2022 Feb 27;13(3):6500–6508. doi: 10.1080/21655979.2022.2033013

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Activation of VEGF/VEGFR2 signaling reduced the effect of PB2 on growth and metastasis of OSCC cells. (a) The viability of SCC-25 cells treated with PB2 with or without VEGF was detected by CCK-8 assay. The apoptosis (B/C) and related proteins (d) in SCC-25 cells treated with PB2 with or without VEGF were respectively analyzed by Tunel assay and Western blot. The migration (e) and invasion (f) of SCC-25 cells treated with PB2 with or without VEGF were detected by wound healing assay and transwell assay. The expression of metastasis associated proteins (g) and EMT related proteins (h) was analyzed by Western.